BIOL 313 (Winter)

Eukaryotic Cell Biology



Instructor(s):
S. Weber
W5/15
(514) 398-2042
steph.weber@mcgill.ca
Workload:
3 credits (3-0-6)
Prerequisite(s):
BIOL 200, BIOL 201 or ANAT 212 / BIOC 212, BIOL 202
Content:
A study of cell biology of eukaryotes focusing on the assembly and function of cellular structures, their relationship to the cell cycle; the dynamics of the cytoskeleton and its motors; mechanics of cell division, cell cycle and checkpoints, nuclear dynamics, chromosome structure and behavior, with a strong emphasis on experimental approaches.

General Course Outline:  

1) ANALYSIS OF CELLULAR PROCESSES
(Chapters  8 and 9; 2 lectures)

  • Genetic, molecular, and biochemical analyses
  • Visualizing cellular processes and individual molecules
  • Microscopy

2) MULTIDISCIPLINARY APPROACH TO A COMPLEX CELLULAR PROCESS: THE NUCLEO-CYTOPLASMIC TRANSPORT (2 lectures)

  • Ultrastructural studies and 3D reconstruction
  • Reconstitution of transport and identification of underlying molecular mechanisms
  • Biochemical/biophysical principles of nuclear transport
  • Ran and the molecular evolution of basic cellular mechanisms

5) THE DYNAMICS OF THE CYTOSKELETON (5 lectures)

  • Microtubules, actin, and intermediate filaments
  • Regulation of cytoskeletal filaments
  • Molecular motors, force generating systems and intracellular motility
  • Integrative mechanisms, molecular and cellular basis of cell migration

6) CELLCYCLE, CHECKPOINTS, APOPTOSIS, CANCER
(Chapters 17 and 23; 7 lectures)


7) MECHANICS OF CELL DIVISION
(Chapter 18; 2 lectures)

  • Molecular mechanisms of mitosis

3) REGULATION OF PROTEIN STABILITY
(2 lectures)

  • Introduction to protein folding, domains and modules
  • Protein turnover, theory and experimental approaches

8) CELL BIOLOGY OF DEVELOPMENT
(Chapter 20 and 21; 4 lectures)

  • Gametogenesis and fertilization
  • Control of embryonic cell divisions and patterning

4) CELL STRUCTURE AND CELL ADHESION
(2 lectures)

  • General introduction to cell structure
  • Epithelial polarity, junctional organization
  • Cell-cell and cell-matrix adhesion


Readings:
Pertinent chapters in Molecular Biology of the Cell, 4th edition, by B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson.
Method:
There are three hours of lectures each week.
Evaluation:

Students will be evaluated on the basis of a one-hour mid-term exam and a cumulative final exam


McGill University values academic integrity. Therefore all students must understand the meaning and consequences of cheating, plagiarism and other academic offences under the Code of Student Conduct and Disciplinary Procedures (see http://www.mcgill.ca/integrity/ for more information).

Last update: March 21, 2017