Dr. Richard ROY
[visit the Roy Lab Website]

As a postdoctoral fellow with Pierre Chambon and Jean-Marc Egly, Richard Roy became involved in the characterization of the basal transcription/DNA repair factor TFIIH. During this work he linked this important protein to an as yet unidentified function in cell cycle regulation. Continuing his interests in the control of cell division, Dr Roy worked with Victor Ambros as a Human Frontiers Fellow where he focused on cell cycle regulation in a developmental context using genetic analysis in C. elegans. Since his appointment at McGill, Dr Roy and his laboratory have developed several novel molecular/genetic links to better understand how cell proliferation is controlled in an organismal context. Work in the Roy Laboratory is funded by the National Cancer Institute of Canada and NSERC. Richard Roy is also currently a CIHR New Investigator, and an Associate Member of the McGill Cancer Centre.

Research in the Roy laboratory
The normal development of an organism depends on the precise orchestration of cell division, differentiation and morphogenesis. Although much is understood about how developmental regulatory genes affect cell differentiation, little is understood about how they control cell proliferation throughout development. Using genetic analysis and molecular and cell biological approaches in C. elegans, the Roy laboratory is engaged in the identification and characterization of both novel and previously known genes which affect cell division throughout the course of embryogenesis and postembryonic development in C. elegans.

A dominant mutation in the general cell cycle regulator Cdc25 causes intestinal cells to multiply out of control during embryogenesis in C. elegans resulting in intestinal hyperplasia in mutant animals. This gene is a suspected oncogene that functions in cell cycle progression and in a critical DNA damage checkpoint. The green highlights the expression of a green fluorescent protein (GFP) reporter transgene that is expressed exclusively in the intestinal epithelium. A. wild-type embryo. B. wild-type C. elegans. C. Cdc25 mutant embryo. D. Cdc25 mutant C. elegans.

Selected Publications

Narbonne P, Roy R. (2009) Caenorhabditis elegans dauers need LKB1/AMPK
to ration lipid reserves and ensure long-term survival. Nature. 457: 210-214.

Hebeisen M, Drysdale J, and Roy R. (2008) Suppressors of the cdc-25.1(gf)-associated intestinal hyperplasia reveal important maternal roles for prp-8 and a subset of splicing factors in C. elegans. The RNA Journal 14: 2618-2633.

Ouellet J, Li S, Roy R. (2008) Notch signalling is required for both dauer maintenance and recovery in C. elegans. Development 135: 2583-2592.

Hebeisen M and Roy R. (2008) CDC-25.1 stability is regulated by distinct domains to restrict cell division during embryogenesis in C. elegans. Development 135: 1259-1269.

Narbonne P and Roy R. (2008) Genes that affect both cell growth and polarity mediate stem cell quiescence. Frontiers in Bioscience 13: 995-1002.

Ouellet J, Roy R. (2007) The lin-35/Rb and RNAi pathways cooperate to regulate a key cell cycle transition in C. elegans. BMC Dev Biol. 7: 38 

Narbonne P., Roy R. (2006) Regulation of germline stem cell proliferation downstream of nutrient sensing. Cell Div. 1(1):29 [Epub ahead of print] 

Kim D.Y., Roy R. (2006) Cell cycle regulators control centrosome elimination during oogenesis in Caenorhabditis elegans. J Cell Biol. Sep 11; 174(6): 751-7.  

Labbe J.C., Roy R. (2006) New developmental insights from high-throughput biological analysis in Caenorhabditis elegans. Clin Genet. Apr; 69(4): 306-14. Review. 

Narbonne P., Roy R. (2006) Inhibition of germline proliferation during C. elegans dauer development requires PTEN, LKB1 and AMPK signalling. Development Feb; 133(4): 611-9.

Kostic, I, Li, S., Roy, R. (2003) cki-1 links cell division and cell fate acquisition in the C. elegans somatic gonad. Developmental Biology 263: 242-252.

Kostic I., Roy R. (2002) Organ-specific cell division abnormalities caused by mutation in a general cell cycle regulator in C. elegansDevelopment 129: 2155-2165.

Hong, Y.*, Roy, R.*, Ambros, V. (1998) Developmental regulation of a cyclin-dependent kinase inhibitor controls postembryonic cell cycle progression in C. elegans. Development 125: 3585-3597.

*equal contribution

Abouheif] [Brouhard] [Dankort] [Dent] [Harrison] [Hekimi]
[Larsson] [Lasko] [Moon] [Nilson] [Roy] [Schöck] [Vogel] [Western] [Zetka]