Sagian Core System

Number

Module

Feature

1

Automated workstation
(Biomek FX, from Beckman Coulter)

- Versatile liquid handler for 96- or 384-well plates.
- Equipped with a 96-channel 200 μl head and a 8-independent channel head.
- Enclosed in an Iso class 5 cleanroom.
- Different units can be installed such as a magnetic bead unit, a filtration unit, a magnetic stirrer, an orbital shaker, a heating unit and a circulating reservoir.

2

Plate shaker (Beckman Coulter)

Configured with 6-plate locations.

3

Plate reader (DTX880, from Beckman Coulter)

- For absorbance, luminescence, fluorescence intensity, fluorescence polarization and time-resolved fluorescence.
- Heated chamber (from 4ºC above ambient up to 45ºC).
- Reads from top or bottom of 6- to 384-well plates.
- Current filter sets include 260, 340, 370, 405, 450, 485, 535, 595 and 620 nm for excitation and 535, 595 and 625 nm for emission.
- Can perform endpoint, multiple wavelength, kinetic, or area scans.

4

Dispenser (Multidrop 384, from Titertek)

8-channel head to dispense 5 to 395 μl from a single or multiple reagent bottles

5

Plate washer
(ELx405 select, from Biotek Instruments)

- Dispenses from top and washes from bottom.
- Flow rate is adjustable for sensitive assays.

6

Lidding station (Beckman Coulter)

- Accommodates up to 6 lids.
- Maintains the lid-plate association.

7

Carousel (Beckman Coulter)

Accommodates up to 90 standard plates and 40 deep-well plates or tip boxes.

8

Robotic arm (ORCA, from Beckman Coulter)

Slides on a 3-meter rail to move plates or labware between devices.

9

Tip lift and disposal unit (Beckman Coulter)

Accommodates up to 96 200 μl tip boxes.

10

Plate sealer (ALPS 300, from Abgene)

Used to seal plates for storage or to prevent evaporation or contamination during incubation or shaking.

11

Thermocycler
(PTC-225 DNA engine tetrad, from MJ Research)

4-independent PCR units.

12

Incubator
(Cytomat 6001, from Kendro)

Accommodates up to 125 standard plates and 30 deep-well plates for incubation from 10 to 50ºC.

Application/Use

- Automation of many steps of lab benchwork

  • DNA miniprep
  • PCR cleanup
  • PCR-genotyping
  • Colorimetric assay
  • Fluorimetric assay
  • Luminescence assay…

- Large-scale screenings: a library of compound will be purchased soon.

The system is programmed and operated by Ming Wang, who also runs a similar system for Dr. G. Bell’s LE3 laboratory

To use the CIAN SAGIAN core system, submit your proposal or contact us

If you need to automate some steps of the benchwork performed in your lab, or plan to run large-scale experiments, contact us. Together with you, we will design, validate and run procedures customized for your project.