What do I provide? |
Three ways to use the plasmid DNA preparation service offered at CIAN: |
Agar plate(s) with single colonies & liquid growth medium supplemented with appropriate antibiotic (1mL/prep) |
OR |
Glycerol stocks in the 96-well format |
OR |
1.5-2mL of overnight cultures of desired clones |
|
How do I submit samples? |
Coordinate with Guillaume Lesage (514-398-5414)
or Ming Wang (514-398-2518 or 5669)
or email at cian@mcgill.ca |
When do I submit samples? |
Any day before 1:30PM |
When can I pick up collection plates? |
Next working day at 4:00PM |
Same day at 4:00PM |
What do I get? |
Two sealed 96-well plates containing (i) 30µL/well of plasmid DNA solution and (ii) 10µL/well bacteria culture |
One sealed 96-well plate containing 30µL/well of plasmid DNA solution |
What are the expected results? |
We routinely obtain 3-6µg DNA (100-200ng/µL, with A260/280 ratio between 1.8-2). DNA quality is compatible for sequencing, PCR or restriction digest |
Which method is used? |
Alkali lysis procedure followed by isopropanol precipitation |
Any quality control? |
To reduce variability and ensure quality, we use commercial solutions and buffers. In addition, a control is included for each batch of preparation, ensuring quality control |
I don’t have 96 samples… Can I use the service? |
Yes. Partial plate can be run at the cost of a full plate
View our rates |