2D/DiGE

Online Booking

Online Registration Form

Fees for 2D/DiGE

Manuals

Manual for DiGE users (GE Heathcare) [PDF format (1.7MB)]
Short instructions for CIAN DiGE users [PDF format (220KB)]
2-D Electrophoresis - Principles and methods (GE Healthcare) [PDF format (247KB)]
2D Western: Cup loading for mini-gel [PDF format (83KB)]
2D Western: Rehydration loading for mini-gel [PDF format (70KB)]
Typhoon User's Mini-manual [PDF format (653 Kb)]
Viewing and editing Typhoon images [PDF format (124Kb)]

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2-D Gel Electrophoresis and DiGE Facilities

What is 2D gel electrophoresis?
What is DiGE?
The advantages of DiGE over traditional 2-D
The advantages of DiGE at CIAN

Equipment
		1st dimension: IEF: IPG-PhorII (GE) and Protean IEF (BioRad)

		2nd dimension: GE Ruby SE600 (16x16cm) GE Ettan DALTsix (26x20cm) BioRad Criterion pre-cast (13x9cm) BioRad Protean (8.6x7cm)

		Data acquisition: GE Typhoon Trio+ Scanner Analysis software: Bio-Rad PDQuest for traditional 2-D gels GE DeCyder for DiGE gels

		Last update: Jan. 11, 2012